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目的 :构建含有野生型DNA聚合酶 β基因的重组表达载体VR10 12 polβ。 方法 :提取胎儿食管上皮组织的总RNA ,反转录成cDNA ,经PCR扩增、酶切后克隆入表达载体VR10 12 ,重组子以小量质粒提取后酶切、PCR、测序鉴定。结果 :插入片段与GenBank报道一致 ,并且插入方向正确。结论 :重组野生型DNA聚合酶 β表达载体VR10 12 polβ的构建成功 ,为深入研究提供了工具
Objective: To construct a recombinant expression vector VR10 12 polβ containing the wild-type DNA polymerase β gene. Methods: The total RNA was extracted from the fetal esophageal epithelium and reverse transcribed into cDNA. The recombinant plasmid was cloned into the expression vector VR1012 after digested by restriction endonuclease. The recombinant plasmid was digested with small amount of plasmid and identified by PCR and sequencing. Results: The inserted fragment was consistent with the GenBank report and the insertion direction was correct. Conclusion: The construction of recombinant wild-type DNA polymerase β expression vector VR10 12 polβ was successful and provided a tool for further study