论文部分内容阅读
目的探讨母婴分离(MS)大鼠脑组织海马区脑源性神经营养因子(BDNF)的变化及其在焦虑调节内脏高敏感中的作用。方法建立MS大鼠模型,模拟肠易激综合征(IBS)内脏高敏感伴焦虑的特征。先后采用旷场实验和腹壁撤退反射(AWR)评估大鼠焦虑行为和内脏敏感性,并分别用酶联免疫吸附方法和免疫组织化学方法检测海马区BDNF的蛋白表达水平;分析MS组大鼠焦虑水平、内脏高敏感程度及海马区BDNF含量之间的相关性。结果①旷场实验中,MS组大鼠中央停留时间及穿越次数少于对照组(P<0.05),提示其焦虑水平增加;②MS组大鼠在直肠扩张20、40、60、80 mmHg时AWR评分均高于对照组(P<0.05);③MS大鼠海马区BDNF含量明显升高(P<0.05),且海马CA1、CA3区BDNF表达阳性细胞数大于对照组(P<0.05);④MS组大鼠焦虑水平、内脏高敏感程度及海马区BDNF蛋白水平两两之间均呈正相关。结论在MS模型模拟的IBS中,脑组织海马区BDNF增高,且参与调节焦虑对内脏高敏感的促进作用。
Objective To investigate the changes of brain-derived neurotrophic factor (BDNF) in the hippocampus and the role of anxiety in the regulation of visceral hypersensitivity in maternal and neonatal rats. Methods MS rat model was established to simulate the characteristics of visceral hypersensitivity with anxiety in irritable bowel syndrome (IBS). Anoxia behavior and visceral sensitivity were evaluated by open field test and abdominal wall reflex (AWR). The protein expression of BDNF in hippocampus was detected by enzyme-linked immunosorbent assay (ELISA) and immunohistochemical method respectively. Anxiety Level, visceral hypersensitivity and BDNF content in hippocampus. Results ① In the open-field test, the median residence time and the number of traversing times in MS group were less than those in control group (P <0.05), suggesting that their anxiety levels were increased. ② In AWMS group, (P <0.05). ③ The content of BDNF in hippocampus of MS rats was significantly increased (P <0.05), and the number of BDNF positive cells in CA1 and CA3 hippocampus was higher than that in control group (P <0.05) There was a positive correlation between anxiety, visceral hypersensitivity and BDNF protein level in hippocampus. Conclusion In IBS simulated by MS model, the BDNF of hippocampus in brain tissue is increased, and it is involved in the regulation of the promotion of visceral hypersensitivity by anxiety.