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选用次代培养的新生金仓鼠肺细胞,首次在体外证实了水不溶性弱致癌物N-苯基-β-萘胺(N-phenyl-β-naphthylamine,PBNA)的瘤性转化活性。细胞经PBNA悬液处理后30-60天显示出恶性细胞的生物学特性,包括失去接触抑制,在体外能无限制生长,出现交叉重叠的转化细胞灶,细胞获得ConA凝集性,在琼脂半固体培养基上生长成集落,有异常(三极分裂、四极分裂)有丝分裂,在免疫抑制的新生小鼠皮下接种后形成瘤结节。扫描电镜示其与对照细胞有不同的形态特征。
The neoplastic cells of neonatal hamster lung cells were selected for the first time in vitro to confirm the neoplastic conversion activity of N-phenyl-β-naphthylamine (PBNA), a water-insoluble weak carcinogen. The cells were treated with PBNA suspension for 30-60 days, showing the biological characteristics of malignant cells, including loss of contact inhibition, unlimited growth in vitro, cross-overlapping transformed cell foci, cell acquisition of ConA agglutination, The culture grew into colonies with abnormal (bipolar, bipolar) mitosis and tumor nodules formed after subcutaneous immunization of immunosuppressed neonatal mice. Scanning electron microscopy showed that it had different morphological characteristics with control cells.