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果树的细胞悬浮培养物是分离原生质体的良好材料，但细胞悬浮培养物的建立需花费大量的精力和较长的时间，而且长期的继代培养还会导致体细胞变异和植株再生能力的下降。因此，研究细胞悬浮培养物的保存方法对原生质体的培养和遗传操作具有重要的意义。超低温保存是长期保存植
Cell suspension culture of fruit trees is a good material for isolating protoplasts, but the establishment of cell suspension cultures requires a lot of energy and time, and long-term subculture also leads to a decrease in somatic and plant regeneration capacity . Therefore, the study of cell suspension culture preservation methods for protoplast culture and genetic manipulation of great significance. Cryogenic preservation is long-term preservation of plants