以蛏子为原料,用酶法提取糖胺聚糖,通过单因素和响应面试验,以糖胺聚糖提取率为指标,优化糖胺聚糖的提取工艺。采用DE-52离子交换层析对糖胺聚糖进行纯化,通过紫外-可见分光光谱和红外光谱分析糖胺聚糖的结构。试验结果表明:酶提取法最佳工艺条件为提取温度51.8℃,酶用量4.2%,酶解时间4.9 h,在此条件下糖胺聚糖得率最大值达2.51%。 Prunus as raw material, enzymatic extraction of glycosaminoglycan, single factor and response surface test to glycosaminoglycan extraction rate as an indicator to optimize the glycosaminoglycan extraction process. Glycosaminoglycans were purified by DE-52 ion exchange chromatography and the glycosaminoglycan structure was analyzed by UV-Vis and IR spectroscopy. The experimental results showed that the optimum extraction conditions were: extraction temperature 51.8 ℃, enzyme dosage 4.2%, enzymolysis time 4.9 h, under this condition the maximum yield of glycosaminoglycan reached 2.51%.