目的:研究不同培养条件对骨骼肌细胞增殖及分化的影响。方法:以体外培养小鼠肌母细胞C2C12为研究模型,对照组用含φ=10%胎牛血清液培养,实验组培养基改变为φ=2%胎牛血清的DMEM液,分别于更换培养液后24、48、72h,观察细胞形态变化。结果:对照组细胞形态实验前后无变化,实验组24、48h时部分细胞出现死亡,72h存活细胞开始融合,肌管形成。结论:胎牛血清含量为φ=2%的DMEM液有促进肌母细胞分化,从而进一步形成肌管的作用;含φ=10%的DMEM不能使之分化,提示不同血清含量的DMEM培养基对体外培养的骨骼肌细胞的增殖和分化有重要影响 Objective: To study the effects of different culture conditions on the proliferation and differentiation of skeletal muscle cells. Methods: Cultured mice myoblasts C2C12 as a model, the control group with φ = 10% fetal bovine serum culture, the experimental group changed to φ = 2% fetal bovine serum DMEM solution, respectively, in the replacement culture After 24, 48, 72h, the changes of cell morphology were observed. Results: The morphological changes of the control group showed no changes before and after the experiment. In the experimental group, some cells died at 24 and 48 hours, and the surviving cells began to fuse at 72 hours and the myotubes formed. CONCLUSION: DMEM with fetal bovine serum level of φ = 2% can promote myoblast differentiation and further myotube formation. DMEM containing φ = 10% can not differentiate, suggesting that DMEM with different serum levels In vitro cultured skeletal muscle cells proliferation and differentiation have a significant impact