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为了解决草菇生产中菇房和灭菌设备不足的困难,我们于1984年进行了露地棉籽壳生料栽草菇试验,收到了较好的效果。现将其工艺流程简介如下。 (一)斜面母种制作:马铃薯200克,蔗糖20克,VB_1 10毫克,MgSO_4 1.5克,KH_2PO_4 3克,琼脂20克,用NaOH液调pH至7～8。称去皮切成小块的马铃薯200克,加水1000ml。煮沸20分钟取滤汁,加琼脂煮溶后加入蔗糖、VB_1等,最后用开水补足至1000ml。分装试管,在1公斤/cm~2压力下灭菌30
In order to solve the straw mushroom production and sterilization equipment shortage of difficulties, we conducted in 1984, cottonseed husk planting grass mushroom test, received good results. Now the process outlined below. (I) Production of bevel mother: 200 grams of potato, 20 grams of sucrose, 10 mg of VB_1, 1.5 grams of MgSO 4, 3 grams of KH 2 PO 4 and 20 grams of agar. The pH value is adjusted to 7-8 with NaOH solution. Said peeled and cut into small pieces of potato 200 grams, add water 1000ml. Boil for 20 minutes to take juice, add agar after cooking add sucrose, VB_1, etc., and finally make up to 1000ml with boiling water. Dispense the test tube and sterilize it at a pressure of 1 kg / cm ~ 2