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目的探讨福建人群DNA损伤修复基因XRCC3 Thr241Met多态与贲门癌、非贲门部胃癌易感性的关系,分析基因与环境因素在癌症发生中的联合作用。方法采用共同对照组的病例对照研究方法,应用PCR-RFLP技术检测基因型,应用非条件logistic回归计算OR值及其95％CI。结果 XRCC3三种基因型(野生型CC、杂合突变型CT、纯合突变型TT)在贲门癌组的分布频率分别为43．2％、46．5％、10．3％;在非贲门部胃癌组的分布频率为53．2％、40．9％、5．8％;在对照组的分布频率分别为59．6％、35．1％、5．3％。经多因素分析结果显示携带变异基因型(CT+TT)个体罹患贲门癌的风险增加(OR=1．76,95％CI:1．07-2．90)。XRCC3变异基因型与饮酒、新鲜蔬菜摄入和慢性胃炎对贲门癌发生存在协同作用;与吸烟、慢性胃炎对非贲门部胃癌发生存在协同作用。结论 XRCC3基因多态与贲门癌发生有关,贲门癌与非贲门部胃癌的危险因素不尽相同。
Objective To explore the relationship between DNA damage repair gene XRCC3 Thr241Met polymorphism and the susceptibility of gastric cardia cancer and noncardia gastric cancer in Fujian population, and to analyze the combined effects of gene and environmental factors in cancer occurrence. Methods The case-control study of the common control group was used to detect the genotypes by PCR-RFLP. The OR and 95% CI were calculated by non-conditional logistic regression. Results The frequencies of XRCC3 genotypes (wild type CC, heterozygous mutant CT, homozygous mutant TT) in the cardiac cancer group were 43.2%, 46.5%, and 10.3%, respectively; The distribution frequency of gastric cancer group was 53.2%, 40.9%, 5.8%; the frequency of distribution in the control group was 59.6%, 35.1%, and 5.3%, respectively. The results of multivariate analysis showed that individuals carrying the variant genotype (CT+TT) had increased risk of cardiac cancer (OR=1.76, 95%CI:1.07-2.90). XRCC3 variant genotype had synergistic effect with alcohol intake, fresh vegetable intake and chronic gastritis on the occurrence of cardiac cancer. There was a synergistic effect with smoking and chronic gastritis on gastric cancer in noncardia gastric cancer. Conclusion The XRCC3 gene polymorphism is associated with the occurrence of cardiac cancer, and the risk factors for cardiac cancer and non-cardia gastric cancer are not the same.