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糖尿病神经病变(Diabetic neuropathy,DN)是糖尿病在神经系统发生的多种并发病变的总称。文章旨在筛选2型糖尿病早期大鼠外周神经节差异表达的基因。采用Illumina大鼠基因表达芯片,比较糖尿病模型与非糖尿病大鼠外周神经节基因表达谱差异。结果表明,全基因组12 604个已知基因中,158个基因差异表达。糖尿病组与非糖尿病组相比,87个基因表达上调,71个表达下调。对差异表达的基因进行GO分析,发现上调基因所参与的最显著(P<0.001)的几个生物学过程都与神经细胞骨架及运动功能有关;下调基因所参与的最显著的生物学过程主要与“对病毒/生物刺激/其它生物的反应”有关。KEGG(Kyoto encyclopedia of genes and genomes)分析显示,差异表达的基因所参与的最显著(P<0.001)的生物学通路为代谢通路。结果表明:高血糖可导致糖尿病大鼠外周神经节代谢紊乱;高血糖可能通过免疫炎症反应、改变神经细胞骨架及运动功能相关的基因的表达,继而损害外周神经节的结构和功能。
Diabetic neuropathy (DN) is a general term for a variety of concurrent pathological changes of diabetes in the nervous system. The purpose of this study was to screen differentially expressed genes in peripheral ganglia of early type 2 diabetic rats. Illumina rat gene expression chips were used to compare the gene expression profiles of peripheral ganglia in diabetic rats and non-diabetic rats. The results showed that of the 12 604 known genes in the whole genome, 158 genes were differentially expressed. Compared with non-diabetic group, 87 genes were up-regulated and 71 down-regulated in diabetic group. GO analysis of the differentially expressed genes found that the most significant (P <0.001) involvement of the up-regulated genes was related to the neuronal cytoskeleton and motor function; the most significant biological processes involved in the down-regulation of genes were mainly And “on the virus / biological stimuli / other biological response ” related. KEGG (Kyoto encyclopedia of genes and genomes) analysis showed that the most significant (P <0.001) biological pathway involved in differentially expressed genes was the metabolic pathway. The results showed that hyperglycemia could lead to disorder of metabolism of peripheral ganglion in diabetic rats. Hyperglycemia might alter the structure and function of peripheral ganglion through immune inflammation reaction and alteration of the expression of nerve cell cytoskeleton and motor function related gene.