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目的:研究羟丙基-β-环糊精(HP-β-CD)包合春砂仁挥发油的最佳工艺,并进行包合物鉴定。方法:以挥发油利用率、包合物含油率与最高含油率比值和包合物收率的综合得分为评价指标,对搅拌法、超声法和研磨法进行优选;以挥发油与HP-β-CD的投料比、HP-β-CD的浓度、包合时间、包合温度为考察因素,上述综合得分为评价指标,采用正交试验优选最佳包合工艺,并通过显微鉴别、差示扫描量热分析、紫外分光光度、气相色谱质谱联用等方法对包合前、后的包合物进行鉴别。结果:最佳包合方法为超声法,最佳包合工艺条件为挥发油与HP-β-CD的投料比为1:10(V:m),HP-β-CD的浓度为40%,包合时间80min,包合温度55℃。包合前、后成分未见明显变化。结论:春砂仁挥发油的HP-β-CD包合工艺切实可行,可为实际生产研究提供一定的试验依据。
OBJECTIVE: To study the best technology of inclusion of hydroxypropyl-β-cyclodextrin (HP-β-CD) in the volatile oil of Amomum villosum and to identify its inclusion compound. Methods: The comprehensive index of volatile oil utilization, the ratio of inclusion oil content to maximum oil content and the inclusion compound yield were used as evaluation indexes to optimize the stirring method, ultrasonic method and grinding method. The volatile oil and HP-β-CD , The concentration of HP-β-CD, the inclusion time and inclusion temperature were investigated. The above comprehensive score was used as the evaluation index. The optimum inclusion process was optimized by orthogonal test, and the results of microscopic identification, differential scanning Calorimetric analysis, UV spectrophotometry, gas chromatography mass spectrometry and other methods before and after inclusion complexes were identified. Results: The best method of inclusion was ultrasonic method. The optimum inclusion conditions were the ratio of volatile oil to HP-β-CD was 1:10 (V: m), the concentration of HP-β-CD was 40% Combined time 80min, inclusion temperature 55 ℃. Inclusion before and after no significant change in composition. Conclusion: HP-β-CD inclusion technology of Volatile Oil of Volatile Oil from Amomum villosum is practicable, which can provide some experimental evidences for the actual production research.