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目的:比较下瘀血汤全药与组分处方抗肝纤维化的作用差异。方法:Wistar大鼠随机分为正常组、造模组。造模组大鼠ip猪血清复制肝纤维化模型,每次0.5 mL/只,每周2次,共12周。造模成功后造模组大鼠随机分为模型组、下瘀血汤组、组分处方组,第8周开始下瘀血汤组、组分处方组大鼠造模同时ig给药,按10 mL.kg-1容积,下瘀血汤组每日2.34 g.kg-1,组分处方组每日0.648 g.kg-1。连续4周。正常组、模型组大鼠ig同体积生理盐水。12周末,处死动物,获取标本,检测血清肝功能、肝脏病理。结果:与模型组相比,下瘀血汤组、组分处方组大鼠肝质量明显减少(P<0.05);肝体比、脾质量、脾体比均有下降趋势;总蛋白(TP),球蛋白(Glb)含量明显减少(P<0.05),A/G比值倒置情况明显改善(P<0.05);白蛋白(Alb)含量均有升高趋势。下瘀血汤组与组分处方组相比较,大鼠肝质量、肝体比、脾质量、脾体比均有好转趋势;Alb含量有升高趋势,TP,Glb含量有下降趋势,A/G比值倒置情况有改善趋势。与正常组相比,模型组、下瘀血汤组、组分处方组纤维化分级差异有显著性(P<0.05);与模型组相比,下瘀血汤组、组分处方组纤维化分级差异有显著性(P<0.05)。提示二者均有一定改善肝功能、改善肝脏病理作用,且下瘀血汤组有优于组分处方组的趋势。结论:降低免疫反应和保护肝细胞是下瘀血汤全药和组分处方抗肝纤维化的主要作用机制,且下瘀血汤全药处方作用优于组分处方。
OBJECTIVE: To compare the effects of prescriptions of Xiaoyu Xuexue decoction on liver fibrosis. Methods: Wistar rats were randomly divided into normal group and model group. Model rats ip pig serum replication of liver fibrosis model, each 0.5 mL / only twice a week for 12 weeks. The model rats were randomly divided into model group, Xiayuxue decoction group, prescription group, and Xiayuxue decoction group at the 8th week. The rats in the formulation prescription group were given ig administration at the same time 10 mL.kg-1 volume, 2.34 g.kg-1 daily in Xiaoyu-Xue-Tang Decoction group and 0.648 g.kg-1 daily in the prescription group. For 4 weeks. Normal group, model group rats ig same volume of normal saline. At the end of the 12th week, animals were sacrificed and specimens were taken for detection of serum liver function and liver pathology. Results: Compared with model group, the liver mass of Xiayuxue decoction group and prescription prescription group decreased significantly (P <0.05), and the ratio of liver mass to body weight, (P <0.05), and the A / G ratio inverted significantly (P <0.05). The content of albumin (Alb) also increased. The liver mass, liver-to-body ratio, spleen mass and spleen ratio in Xia Yu Xue Tang Decoction group were better than those in the prescription group. Alb content increased, TP and Glb content decreased, while A / G ratio inversion situation to improve the trend. Compared with the normal group, there were significant differences in the grading of fibrosis between the model group, Xiayuxue decoction group and the prescription prescription group (P <0.05). Compared with the model group, Xiayu Decoction group, Grading differences were significant (P <0.05). These results suggest that both of them have a certain improvement in liver function and liver pathology, and that of Xiayu Decoction group is better than that of the prescription group. Conclusion: It is the main mechanism of anti-hepatic fibrosis that the immune response and the protection of liver cells are the prescriptions of Xiayuxuexue decoction.