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本实验以C57BL/6J小鼠为实验对象,通过建立高血脂模型,喂食不同剂量的岩藻黄素微囊粉,测定了小鼠血清和肝脏中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的含量,并利用RT-PCR法探讨了岩藻黄素微囊粉对小鼠体内胆固醇代谢的调控作用机制。结果表明岩藻黄素微囊粉能有效降低肝脏中的TC、TG和LDL-C的含量,增加HDL-C的含量;降低血清中TG的含量,增加清血清中HDL-C的含量,同时增加了小鼠粪便中粗脂肪和胆固醇的含量,但在代谢过程中却并没有降低血清中的TC含量。RT-PCR结果显示岩藻黄素微囊粉调节胆固醇代谢的作用机制可能与其能够抑制HMG-COA-R、CYP7A1、LXRα、ABCA1和ACAT2等基因的表达,促进SREBP2、LCAT以及LDLR等基因的表达有关。
In this experiment, C57BL / 6J mice were used as experimental subjects. The levels of total cholesterol (TC), triglyceride (TG) and high triglyceride (TG) in mice serum and liver were measured by establishing hyperlipidemia model and feeding different doses of fucoxanthin microcapsule powder Density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C), and the mechanism of the effect of fucoxanthine microcapsule powder on the cholesterol metabolism in mice was investigated by RT-PCR. The results showed that fucoxanthine microcapsule powder can effectively reduce the content of TC, TG and LDL-C in the liver, increase the content of HDL-C, decrease the content of TG in serum and increase the content of HDL-C in serum, The content of crude fat and cholesterol in the feces of mice, but did not reduce the TC content in the serum during the metabolic process. RT-PCR results showed that the mechanism of fucoidan microcapsules regulating cholesterol metabolism may be related to its ability to inhibit the expression of genes such as HMG-COA-R, CYP7A1, LXRα, ABCA1 and ACAT2 and promote the expression of genes such as SREBP2, LCAT and LDLR.