论文部分内容阅读
背景与目的:胶质瘤治疗效果差,是神经外科领域研究的难点。本研究建立正常免疫(Balb/c小鼠)、T细胞免疫缺陷(裸小鼠)和补体功能缺陷(补体C3基因敲除小鼠)三种不同免疫背景的小鼠G422胶质瘤颅内移植瘤模型,并观察肿瘤的生长特点。方法:将小鼠源性G422胶质瘤细胞种植入Balb/c小鼠、裸小鼠和补体C3基因敲除小鼠脑内,观察肿瘤种植后三种小鼠的生存期、成瘤率、肿瘤生长情况及病理学特性。结果:Balb/c鼠、裸小鼠、补体C3基因敲除小鼠三种小鼠脑内种植G422胶质瘤细胞后,成瘤率分别为90%、100%、100%。Balb/c荷瘤鼠平均生存期最长[(44.3±6.0)d],裸小鼠次之[(24.8±4.8)d],补体C3基因敲除小鼠最短[(18.6±5.2)d],肿瘤体积增大至34.29mm3需要的时间分别为35d、21d、14d;组织病理学观察显示G422胶质瘤细胞脑内种植后可保持胶质瘤的肿瘤特征。结论:采用G422小鼠胶质瘤细胞种植入Balb/c小鼠、裸小鼠、补体C3基因敲除小鼠脑内,可建立具有不同免疫背景的胶质瘤动物模型。
Background and Objective: The poor therapeutic effect of glioma is a difficult point in the field of neurosurgery. In this study, intradermal transplantation of mouse G422 glioma was established in three different immune backgrounds, normal immune (Balb / c mice), T cell immunodeficiency (nude mice) and complement dysfunction (complement C3 knockout mice) Tumor model, and observe the growth characteristics of the tumor. METHODS: Mouse-derived G422 glioma cells were implanted into the brains of Balb / c mice, nude mice and complement C3 knockout mice. The survival, tumor formation rate, Tumor growth and pathological features. Results: The tumorigenic rates of Balb / c mice, nude mice and complement C3 knockout mice were 90%, 100% and 100%, respectively. The mean survival time of Balb / c mice was the highest (44.3 ± 6.0 days), followed by that of nude mice (24.8 ± 4.8 days) and the shortest [(18.6 ± 5.2) days) of complement C3 knockout mice , Tumor volume increased to 34.29mm3 the time required for 35d, 21d, 14d; histopathological observation showed that G422 glioma cells in the brain can maintain glioma tumor characteristics. Conclusion: G422 mouse glioma cells were implanted into Balb / c mice, nude mice and complement C3 knockout mice. Animal models of gliomas with different immune backgrounds were established.