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目的研究并优化类风关巴布剂中雷公藤甲素的提取方法,并测定其含量。方法 HPLC测定类风关巴布剂中的雷公藤甲素含量,采用正交试验设计法考察甲醇浓度、甲醇体积、浸渍时间、提取时间对雷公藤甲素提取率的影响。结果最佳提取条件为用100 mL的60%甲醇,浸渍4 h,加热回流2 h提取效率最高。结论本方法为类风关巴布膏的质量控制提供了可靠的方法。
OBJECTIVE To study and optimize the extraction method of triptolide in the wind-related cataplasm and determine its content. Methods The content of triptolide in wind-air-cataplasm was determined by HPLC. The effects of methanol concentration, methanol volume, immersion time and extraction time on the extraction rate of triptolide were investigated by orthogonal design. Results The optimum extraction conditions were as follows: 100 mL of 60% methanol was used for 4 h, and the extraction efficiency was the highest when it was heated for 2 h. Conclusion This method provides a reliable method for the quality control of albuginea.