对灰树花进行了液体深层培养试验，结果表明：液体培养基碳源以豆粉＋麸皮、玉米粉＋麸皮和氮源以蛋白胨、牛肉膏等较适宜菌丝生长的物质，确定了灰树花深层培养的液体培养基配方；并研究了培养条件对菌丝生物量的影响，得出了培养液ｐＨ在５３０～６００和通气量为１：（１００～１４０）的条件下，菌丝生物量达到最大 The liquid deep culture experiment of Grifola frondosa was carried out. The results showed that the carbon source of liquid culture medium was composed of soybean powder + bran, corn flour + wheat bran and nitrogen source with more suitable mycelial growth, such as peptone and beef extract Grifola frondosa deep liquid culture medium formula; and studied the culture conditions on mycelial biomass, obtained the culture medium pH 530 ~ 600 and ventilation 1: (1  00 ~ 1  40) under the conditions of mycelial biomass to achieve the maximum