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目的观察葛根甘草复合制剂对酒精性肝损伤的预防作用。方法采用酒精灌胃制备酒精性肝损伤大鼠模型。SD雄性大鼠60只,随机分为6组,分别为肝损伤模型对照组、空白对照组、阳性对照组及复合制剂的低、中、高3个剂量组。各剂量组每天经口灌胃给予复合制剂,空白对照组和模型对照组给予蒸馏水,阳性对照组给予海王牌金樽片。于实验的第30天各组大鼠一次性经口给予50%乙醇12mL/kg BW(空白对照组动物给蒸馏水),禁食16h处死动物,剖腹取肝,制备肝匀浆。测定肝组织中的丙二醛(MDA)、谷光甘肽(GSH)和甘油三酯(TG)含量并进行病理组织学检查。结果与模型对照组比较,复合制剂5.0mL/kg、15.0mL/kg剂量组能明显降低酒精性肝损伤大鼠肝组织中MDA含量,复合制剂15.0mL/kg剂量组能明显降低肝损伤大鼠肝组织中的TG含量、升高GSH含量,并能改善肝细胞脂肪变性。结论葛根甘草复合制剂对大鼠酒精性肝损伤有预防作用。
Objective To observe the preventive effect of Pueraria licorice compound on alcoholic liver injury. Methods Alcohol-induced gastric injury was induced in rats by alcohol gavage. Sixty male Sprague-Dawley rats were randomly divided into 6 groups: low, middle and high dosage groups of liver injury model control group, blank control group, positive control group and compound preparation. The rats in each dose group were given orally gavage daily with distilled water, and the blank control group and model control group were given distilled water. On the 30th day of the experiment, rats in each group were orally administered 50% ethanol 12mL / kg BW once a day (blank control group animals were given distilled water), animals were sacrificed 16h after fasting, liver was harvested by laparotomy to prepare liver homogenate. The content of malondialdehyde (MDA), glutathione (GSH) and triglyceride (TG) in liver tissue were determined and histopathological examination was performed. Results Compared with the model control group, the compound preparation 5.0 mL / kg and 15.0 mL / kg significantly decreased the content of MDA in liver tissue of alcoholic liver injury rats, and the compound preparation 15.0 mL / kg dose group could obviously reduce the hepatic injury rats Liver content of TG, increased GSH content, and can improve fatty degeneration of liver cells. Conclusion Pueraria licorice compound preparation can prevent alcoholic liver injury in rats.