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目的 观察半乳糖化白蛋白磁性阿霉素纳米粒在正常肝脏中的靶向性 ,并观察半乳糖化白蛋白磁性阿霉素纳米粒在全身各脏器的分布特征及外加磁场对其分布的影响。方法 大鼠正中开腹 ,肝动脉插管并固定 ,肝动脉注射12 5I半乳糖化白蛋白磁性阿霉素纳米粒 (相当于阿霉素0 .5mg/kg) ,左外叶加磁场 ,磁场应用 3 0min ,移去磁场后 ,动物立即处死 ;对照组 :肝动脉注射半乳糖化白蛋白磁性阿霉素纳米粒 ,左外叶不加磁场 ,3 0min后 ,移去磁场后 ,动物立即处死 ,立即取靶区肝、非靶区肝、肾、心、肺、小肠、脾及周围血作γ计数。肝组织作病理切片。结果 注入的纳米粒75 %～ 85 %分布于肝脏 ,其他脏器极少。病理切片显示磁区小动脉见大量纳米粒存在 ,对照组及非磁区肝中纳米粒很少见。结论 半乳糖化白蛋白磁性阿霉素纳米粒在正常肝组织中有明显的磁靶向性 ;在磁场作用下 ,半乳糖化白蛋白磁性阿霉素纳米粒主要分布于肝脏 ,其他脏器含量很少 ;实验组肾、心、肺、小肠、脾及外周血与对照组的放射活性比较明显降低 ,表明磁性物质的存在使这些脏器的相对药物暴露明显减少
Objective To observe the targeting of galactosylated albumin magnetic doxorubicin nanoparticles in normal liver and to observe the distribution characteristics of galactosylbumin magnetic doxorubicin nanoparticles in various organs and their distribution influences. Methods The rats were enrolled in the middle and the hepatic artery was cannulated and fixed. The hepatic artery was injected with 125I galactosylbumin magnetic doxorubicin nanoparticles (equivalent to 0.5 mg / kg doxorubicin), left external magnetic field and magnetic field The animals were sacrificed immediately after removal of the magnetic field with the application of 30 min. The control group: Hepatic artery was injected with magnetic adriamycin-loaded galactosylbumin nanoparticles, and the left outer lobe was devoid of magnetic field. After 30 minutes, the animals were sacrificed immediately after removing the magnetic field , Immediately take the target area of liver, non-target area of liver, kidney, heart, lung, small intestine, spleen and peripheral blood for γ count. Liver tissue for biopsy. Results The injected nanoparticles ranged from 75% to 85% in the liver, with very few other organs. Pathological sections showed a large number of magnetic artery area to see the presence of a large number of nanoparticles, the control group and non-magnetic nanoparticles in the liver is rare. Conclusions Galactosylated albumin magnetic doxorubicin nanoparticles have obvious magnetic targeting in normal liver tissue. Under the action of magnetic field, galactosylated albumin magnetic doxorubicin nanoparticles are mainly distributed in the liver, and the contents of other organs Very little; experimental group kidney, heart, lung, small intestine, spleen and peripheral blood compared with the control group significantly reduced the radioactivity, indicating that the presence of magnetic substances so that these organs relative drug exposure decreased significantly