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本实验探讨用BA－ELISA方法检测培养的平滑肌细胞清道夫受体的方法。第10～15代平滑肌细胞接种于96孔板，加入氧化低密度脂蛋白培养一定时间，固定，按BA－ELISA法测定细胞结合及摄入氧化低密度脂蛋白的量，以反映清道夫受体的活性。结果表明，随培养时间延长至6～24h，清道夫受体活性已明显可测。说明第10～15代的平滑肌细胞在一定条件下可表达清道夫受体活性。
This experiment was to explore the method of detecting the scavenger receptor of cultured smooth muscle cells by the method of BA-ELISA. The 10th to 15th generation of smooth muscle cells were seeded in 96-well plates, cultured with oxidized low-density lipoprotein for a certain period of time, fixed, and the amount of oxidized low-density lipoproteins was measured by BA-ELISA to reflect the scavenger receptor Activity. The results showed that with the incubation time extended to 6 ~ 24h, scavenger receptor activity has been clearly measurable. Smooth muscle cells of the 10th to 15th generation could express scavenger receptor activity under certain conditions.