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目的观察软肝冲剂对四氯化碳(CCl4)诱导的小鼠肝纤维化模型的保护作用。方法将60只C57B/L小鼠随机分为空白对照组、模型组、秋水仙碱对照组、软肝冲剂低剂量组和软肝冲剂高剂量组,每组12只。空白对照组腹腔注射100%花生油1 ml/kg,每周2次,共10周;其余各组均给予腹腔注射含20%CCl4的花生油1 ml/kg,每周2次,共10周造模;第3周起秋水仙碱对照组予秋水仙碱0.1 mg/(kg·d)灌胃,软肝冲剂低、高剂量组分别予软肝冲剂1 g/(kg·d)、5 g/(kg·d)灌胃。在第10周通过摘除眼球取血,检测小鼠血清中透明质酸(HA)、层粘连蛋白(LN)、三型前胶原(PCⅢ)、四型胶原(Ⅳ-C)、丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)含量,检测肝脏组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,计算肝脏指数,观察小鼠肝脏的HE染色病理组织学改变。结果模型组小鼠血清中HA、LN、PCⅢ、Ⅳ-C、ALT和AST含量分别为(480.11±62.12)μg/L、(411.65±30.63)μg/L、(454.12±41.81)μg/L、(311.15±31.61)μg/L、(140.4±9.8)U/L和(198.2±9.7)U/L,均明显高于其它各组(P<0.05);肝脏组织中SOD[(122.53±7.68)U/mg]低于空白对照组(P<0.05);MDA[(5.59±0.33)nmol/mg]高于空白对照组(P<0.05);肝脏指数[(4.7±1.5)%]高于空白对照组(P<0.05)。病理切片显示肝细胞坏死并出现脂肪空泡、纤维组织增生和炎细胞浸润。软肝冲剂低剂量组与秋水仙碱组治疗效果相似。软肝冲剂高剂量组效果较好,小鼠血清中HA、LN、PCⅢ、Ⅳ-C、ALT和AST含量分别为(143.21±21.53)μg/L、(139.11±35.23)μg/L、(134.45±24.77)μg/L、(119.32±29.34)μg/L、(45.4±6.3)U/L和(53.6±7.1)U/L,均明显低于模型组(P<0.05);肝脏组织中SOD[(189.5±7.5)U/mg]高于模型组(P<0.05),MDA[(2.45±0.33)nmol/mg]低于模型组(P<0.05);肝脏指数[(3.4±0.4)%]低于模型组(P<0.05);病理检查显示肝脏组织中无炎性浸润、脂肪空泡和无纤维组织沉积。结论软肝冲剂可以改善肝脏功能,保护肝细胞和抑制肝纤维化,减轻CCl4诱导小鼠肝纤维化程度。
Objective To observe the protective effect of Ruangan granules on carbon tetrachloride (CCl4) -induced liver fibrosis in mice. Methods Sixty C57B / L mice were randomly divided into blank control group, model group, colchicine control group, Ruanghuang granules low dosage group and Ruangan granules high dosage group, 12 rats in each group. The blank control group was given intraperitoneal injection of 100% peanut oil 1 ml / kg twice a week for 10 weeks. The other groups were given intraperitoneal injection of peanut oil containing 20% CCl4 1 ml / kg twice a week for 10 weeks. From the third week, the colchicine control group was treated with colchicine 0.1 mg / (kg · d) orally, and Ruangan granules at low dosage and high dosage group were given respectively at 1 g / (kg · d), 5 g / (kg · d) Gavage. At the tenth week, blood was taken from the eyeballs and the serum levels of HA, LN, PCⅢ, IV-C, (ALT) and aspartate aminotransferase (AST) were measured. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver tissue were detected to calculate the liver index. The histopathological changes in the liver of the mice were observed . Results The serum levels of HA, LN, PCⅢ, Ⅳ-C, ALT and AST in the model group were (480.11 ± 62.12) μg / L and (411.65 ± 30.63) μg / L and (454.12 ± 41.81) μg / (311.15 ± 31.61) μg / L, (140.4 ± 9.8) U / L and (198.2 ± 9.7) U / L respectively, which were significantly higher than those in other groups (P < (5.59 ± 0.33) nmol / mg] was higher than that of the blank control group (P <0.05); the liver index [(4.7 ± 1.5)%] was higher than that of the blank control group Control group (P <0.05). Pathological sections showed hepatocellular necrosis and the appearance of fat vacuoles, fibrous tissue proliferation and inflammatory cell infiltration. Ruanghuang granules low-dose group and colchicine group treatment effect is similar. The content of HA, LN, PCⅢ, Ⅳ-C, ALT and AST in mice serum was (143.21 ± 21.53) μg / L, (139.11 ± 35.23) μg / L and ± 24.77μg / L, (119.32 ± 29.34) μg / L, (45.4 ± 6.3) U / L and (53.6 ± 7.1) U / L respectively) were significantly lower than those in the model group MDA level was significantly lower than that of model group (P <0.05); [(189.5 ± 7.5) U / mg] ] Were lower than those in the model group (P <0.05). Pathological examination showed no inflammatory infiltration, fat vacuoles and no fibrous tissue deposition in the liver tissue. Conclusion Ruangan granules can improve liver function, protect liver cells and inhibit liver fibrosis and reduce the degree of liver fibrosis induced by CCl4 in mice.