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目的:探讨胰岛素自身抗体(IAA)、胰岛细胞抗体(ICA)及谷氨酸脱羧酶抗体(GADA)联合检测对糖尿病分型的诊断价值。方法:选择2015年6月~2016年6月在我院进行诊治的1型糖尿病患者30例为A组,2型糖尿病患者60例为B组,同期在我院进行体检健康者50例为C组,采用酶联免疫吸附法(ELISA)检测三组的IAA、ICA及GADA,比较三组的阳性检出率。结果:A组空腹血糖为(10.12±3.68)mmol/L,B组空腹血糖为(11.23±3.26)mmol/L,A组和B组的空腹血糖均明显高于C组(4.35±1.42mmol/L)(P<0.05),但A组和B组的空腹血糖相比无明显差异(P>0.05);A组和B组的IAA、ICA及GADA单独和联合检测的阳性率均明显高于C组(P<0.05),且A组的IAA、ICA及GADA单独和联合检测的阳性率明显高于B组(P<0.05);IAA、ICA及GADA联合检测对1型和2型糖尿病的敏感性和特异性均明显高于单独检测(P<0.05)。结论:IAA、ICA及GADA联合检测对糖尿病分型具有较高的临床诊断价值。
Objective: To investigate the diagnostic value of the combined detection of insulin autoantibodies (IAA), islet cell antibodies (ICA) and glutamic acid decarboxylase antibody (GADA) in the diagnosis of diabetic patients. Methods: Thirty patients with type 1 diabetes mellitus who were diagnosed and treated in our hospital from June 2015 to June 2016 were selected as group A, 60 patients with type 2 diabetes as group B, and 50 patients who were examined in our hospital during the same period as C Group. The IAA, ICA and GADA of the three groups were detected by enzyme-linked immunosorbent assay (ELISA). The positive detection rate was compared between the three groups. The fasting blood glucose of group A and group B was significantly higher than that of group C (4.35 ± 1.42 mmol / L, P <0.01) (P <0.05). However, there was no significant difference in fasting blood glucose between group A and group B (P> 0.05). The positive rates of IAA, ICA and GADA alone and in combination in group A and group B were significantly higher than those in group B (P <0.05). The positive rates of IAA, ICA and GADA in group A and group B were significantly higher than those in group B (P <0.05). The detection rates of IAA, ICA and GADA in type 1 and type 2 diabetes mellitus Sensitivity and specificity were significantly higher than the single test (P <0.05). Conclusion: The combined detection of IAA, ICA and GADA has a high clinical value in the diagnosis of diabetes.