A lab-incubation experiment was conducted to investigate the effects of different forms of nitrogen application (ammonium, NH4+-N; nitrate, NO3--N; and amide-N, NH2-N) and different concentrations (40, 200 and 800 mg L-1) on N2O emission from the fluvo-aquic soil subjected to a freezing-thawing cycling. N2O emission sharply decreased at the start of soil freezing, and then showed a smooth line with soil freezing. In subject to soil thawing, N2O emission increased and reached a peak at the initial thawing stage. The average N2O emissions with addition of NH4+-N, NO3--N and NH2-N are 119.01, 611.61 and 148. 22 μg m-2 h-1, respectively, at the concentration of 40 mg L-1; 205.28, 1 084.40 and 106.13 μg m-2 h-1 at the concentration of 200 mg L-1; and 693.95, 1 820.02 and 49.74 μg m-2 h-1 at the concentration of 800 mg L-1. The control is only 100.35 μg m-2 h-1. N2O emissions with addition of NH4+-N and NO3--N increased with increasing concentration, by ranging from 17.49 to 425.67% for NH4+-N, and from 563.38 to 1 458.6% for NO3--N compared with control. There was a timelag for N2O emission to reach a steady state with an increase of concentration. In contrast, by adding NH2-N to soil, N2O emission decreased with increasing concentration. In sum, NH4+-N or NO3--N fertilizer incorporated in soil enhanced the cumulative N2O emission from the fluvo-aquic soil relative to amide-N. This study suggested that ammonium and nitrate concentration in overwintering water should be less than 200 and 40 mg L-1 in order to reduce N2O emissions from soil, regardless of amide-N. A lab-incubation experiment was conducted to investigate the effects of different forms of nitrogen application (ammonium, NH4 + -N; nitrate, NO3- N; and amide-N, NH2- L-1) on N2O emission from the fluvo-aquic soil subjected to freezing-thawing cycling. N2O emission sharply decreased at the start of soil freezing, and then showed a smooth line with soil freezing. increased and reached a peak at the initial thawing stage. The average N2O emissions with addition of NH4 + -N, NO3 - N and NH2-N are 119.01, 611.61 and 148. 22 μg m-2 h-1, respectively, at the concentration of 40 mg L-1; 205.28, 1 084.40 and 106.13 μg m-2 h-1 at the concentration of 200 mg L-1; and 693.95, 1 820.02 and 49.74 μg m-2 h-1 at the concentration of 800 mg L-1. The control is only 100.35 μg m-2 h-1. N2O emissions with addition of NH4 + -N and NO3 - N increased with increasing concentration, by ranging from 17.49 to 425.67% for NH4 + -N, and from 563.38 to 1 458.6% for NO3 - N compared with control. There was a timelag for N2O emission reaching reach steady state with an increase of concentration. In contrast, by adding NH2-N to soil, N2O In sum, NH4 + -N or NO3 - N fertilizer incorporated in soil enhanced the cumulative N2O emission from the fluvo-aquic soil relative to amide-N. This study suggests that ammonium and nitrate concentration in overwintering water should be less than 200 and 40 mg L-1 in order to reduce N2O emissions from soil, regardless of amide-N.