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Electrospray mass spectrometry investigation shows that denatured oxidized insulin B chain can be selectively cleaved by simple Cu(II) ion and the site of cleavage is at Gly8 - Ser9 bond which is second amide bond left from His 10 in the sequence of oxidized insulin B chain.
Electrospray mass spectrometry investigation that that denatured oxidized insulin B chain can be selectively cleaved by simple Cu (II) ion and the site of cleavage is at Gly8 - Ser9 bond which is second amide bond left from His 10 in the sequence of oxidized insulin B chain .