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目的:研究血管紧张素Ⅱ-1型受体(AT1R)基因A1166C多态性与新疆哈萨克族原发性高血压(EH)的相关关系。方法:采用多聚酶链式反应法及限制性片段长度多态性技术(PCR-RFLP),对新疆哈萨克族321例EH患者(EH组)及203例正常血压者(对照组)的外周血白细胞DNA,进行AT1R基因A1166C多态性检测,统计分析该位点不同基因型及等位基因频率在EH组和对照组中的分布。结果:AA、AC和CC基因型在EH组的分布频率为0.7664、0.2274和0.0062,在对照组中分别为0.7537、0.2463和0,2组对比差异无统计学意义(P>0.05);A1166与1166C等位基因频率在EH组中分别为0.8801、0.1199,在对照组中分别为0.8768和0.1232,2组相比差异也无统计学意义(P>0.05)。结论:AT1R基因A1166C多态位点分子变异与新疆哈萨克族EH易感可能不相关。
Objective: To study the relationship between A1166C polymorphism of angiotensin Ⅱ type 1 receptor (AT1R) gene and essential hypertension (EH) in Kazakstan in Xinjiang Uygur Autonomous Region. Methods: Polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were used to detect the peripheral blood leukocyte DNA in 321 EH patients (EH group) and 203 normal subjects (control group) , A1166C AT1R gene polymorphism detection, statistical analysis of the site of different genotypes and allele frequencies in the EH group and control group distribution. Results: The distribution frequencies of AA, AC and CC genotypes in EH group were 0.7664, 0.2274 and 0.0062, respectively, there were no significant differences between the two groups (0.7537, 0.2463 and 0, 2) (P> 0.05) The frequency of 1166C allele in EH group was 0.8801,0.1199, respectively. There was no significant difference between the two groups in the control group (0.8768 and 0.1232, P> 0.05). Conclusion: The molecular variation of AT1R gene A1166C polymorphism site may not be related to the EH susceptibility of Kazak in Xinjiang.