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目的观察血管紧张素1-7(Ang1-7)对大鼠脑胶质瘤C6细胞增殖及与增殖密切相关的磷酸化细胞外信号调节激酶(p-ERK)表达的影响。方法用选择性表达Ang1-7受体Mas的C6细胞作为脑胶质瘤细胞模型,实验分为3组:正常对照组、Ang1-7处理组、A779联合Ang1-7处理组。正常对照组:未经药物处理;Ang1-7处理组:用含1×10-6mol·L-1Ang1-7的培养基处理细胞;A779联合Ang1-7处理组:用同时含有1×10-5mol·L-1A779和1×10-6mol·L-1Ang1-7的培养基处理细胞。用噻唑蓝法检测细胞增殖,用Western印迹法分析p-ERK/ERK表达变化。结果 Ang1-7能够抑制C6细胞增殖,抑制细胞内p-ERK/ERK的水平。A779有拮抗Ang1-7的作用。结论 Ang1-7可能通过Mas受体抑制p-ERK表达,对C6细胞增殖有抑制作用。
Objective To investigate the effects of angiotensin 1-7 (Ang1-7) on the proliferation and the expression of phosphorylated extracellular signal-regulated kinase (p-ERK) in rat C6 glioma cells. Methods C6 cells, which selectively express Ang1-7 receptor Mas, were used as a model of glioma cells. The experiment was divided into 3 groups: normal control group, Ang1-7 treated group, A779 combined with Ang1-7 treated group. Normal control group: without drug treatment; Ang1-7 treatment group: cells treated with 1 × 10-6mol·L-1Ang1-7 medium; A779 combined with Ang1-7 treatment group: treated with 1 × 10-5mol Cells were treated with L-1A779 and 1 × 10-6 mol·L-1Ang1-7 medium. Cell proliferation was measured by thiazolyl blue staining and the expression of p-ERK / ERK was analyzed by Western blotting. Results Ang1-7 could inhibit the proliferation of C6 cells and inhibit the level of p-ERK / ERK in cells. A779 antagonizes Ang1-7. Conclusion Ang1-7 may inhibit the expression of p-ERK through Mas receptor and inhibit the proliferation of C6 cells.