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目的:探讨化红胶囊对去势大鼠骨质疏松的影响及作用机制。方法:取3月龄SD雌性大鼠经去势手术或者是假阳性手术后,喂以去大豆特殊饲料7周以诱导骨丢失。经去势手术大鼠进一步分为化红胶囊组(1.0,0.5,0.25 g.kg-1)、模型组、己烯雌酚组,连续给药12周。检测大鼠血清的骨钙素、雌二醇、尿中脱氧吡啶啉含量,以及椎骨末端骨小梁的变化情况。结果:化红胶囊能降低大鼠血清中骨钙素以及尿液中脱氧吡啶啉含量,提高血清中雌二醇含量,与模型组相比,1.0g.kg-1,0.5 g.kg-1剂量组差异有显著性(P<0.05或P<0.01)。骨小梁的形态学观察及数据分析显示,与模型组相比,化红胶囊可以提高骨小梁面积百分比,与模型组相比,1.0,0.5 g.kg-1剂量组差异有显著性(P<0.05或P<0.01)。结论:化红胶囊可以降低去势大鼠骨转换率和骨吸收程度,提高骨小梁平均面积百分比,是一种潜在的治疗绝经后骨质疏松药物,其作用机制之一可能与化红胶囊雌激素样作用有关。
Objective: To investigate the effect of Huahong capsule on osteoporosis in ovariectomized rats and its mechanism. Methods: Three-month-old SD female rats were castrated or false-positive surgery and fed with special soy-based diet for 7 weeks to induce bone loss. The castrated rats were further divided into Huazhong capsule group (1.0,0.5,0.25 g.kg-1), model group, diethylstilbestrol group, continuous administration for 12 weeks. Serum levels of osteocalcin, estradiol, deoxypyridinum in urine and the changes of trabecular bone at the distal vertebrae were detected. Results: Huahong Capsule can reduce serum osteocalcin and urine deoxy-pyridine content, increase serum estradiol levels, compared with the model group, 1.0g.kg-1, 0.5 g.kg-1 There was significant difference between dose group (P <0.05 or P <0.01). Trabecular morphology and data analysis showed that compared with the model group, Huahong capsule can increase the trabecular area percentage, compared with the model group, 1.0,0.5 g.kg-1 dose group was significantly different ( P <0.05 or P <0.01). CONCLUSION: Huahong capsule can reduce the bone turnover rate and bone resorption in ovariectomized rats and increase the average trabecular area percentage. It is a potential drug for the treatment of postmenopausal osteoporosis. One of the mechanisms may be that Huahong capsule Estrogen-like effects.