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目的 :观察比较混合脐血血浆和成人 AB血清在 CD3AK细胞培养中的作用效果。方法 :分别用混合脐血血浆和成人 AB血清培养 CD3AK细胞 ,检测两组细胞的增殖特性、杀伤活性及免疫表型。结果 :混合脐血血浆组的增殖倍数略低于血清组 ,对 K5 6 2、HL - 6 0的杀伤活性则稍高于血清组 ,但差异均无显著性意义 (均为 P >0 .0 5 ) ;两组的免疫表型都以 CD3+、CD8+为主 ,亦无显著性差异 ( P >0 .0 5 )。结论 :混合脐血血浆在 CD3AK等免疫效应细胞的培养中可能和成人 AB血清具有相似的效果
Objective: To observe the effect of mixed cord blood plasma and adult AB serum in CD3AK cell culture. Methods: CD3AK cells were cultured with mixed cord blood and adult AB serum respectively. Proliferation, cytotoxicity and immunophenotype of the two groups of cells were detected. Results: The proliferation of mixed cord blood group was slightly lower than that of serum group, and the killing activity to K5 6 2 and HL - 60 was slightly higher than that of serum group (all P> 0. 0 5). The immunophenotypes of both groups were predominantly CD3 + and CD8 +, with no significant difference (P> 0.05). CONCLUSION: Mixed cord blood plasma may have similar effects as adult AB serum in culturing immune effector cells such as CD3AK