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目的 :探讨NADPH -细胞色素C还原酶 (CR)在Graves病甲状腺过氧化氢 (H2 O2 )生物合成中作用。方法 :应用高香草酸荧光分析等技术测定Graves病和正常甲状腺H2 O2 浓度和CR活性 ,观察CR活性变化对H2 O2 合成的影响。结果 :Graves病甲状腺CR活性和H2 O2 水平均明显高于正常而H2 O2 酶活性无改变。加CR抑制剂对氯汞苯甲酸后 ,Graves病和正常甲状腺CR活性降低 84% ,同时H2 O2 水平下降近 5 7%。H2 O2 水平随CR活性降低而降低 ,两者呈显著正相关。结论 :CR参与Graves病甲状腺内H2 O2 生物合成 ,是甲状腺内H2 O2 生成的重要途径。
Objective: To investigate the role of NADPH - cytochrome C reductase (CR) in thyroid hydrogen peroxide (H2O2) biosynthesis in Graves disease. Methods: High vanillic acid fluorescence assay was used to determine the H2O2 concentration and CR activity in Graves’ disease and normal thyroid gland, and the effect of CR activity on H2O2 synthesis was observed. Results: Graves’ disease thyroid CR activity and H2O2 levels were significantly higher than normal and H2O2 enzyme activity was unchanged. After adding CR inhibitor p-chloromercuribenzoate, the activity of CR in Graves’ disease and normal thyroid decreased by 84%, while H2O2 level decreased by nearly 57%. H 2 O 2 level decreased with the decrease of CR activity, and there was a significant positive correlation between them. CONCLUSION: CR participates in H2O2 biosynthesis in the thyroid gland of Graves’ disease and is an important pathway of H2O2 production in thyroid.