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比较不同来源姜黄提取物对水杨酸、间羟基苯甲酸、对羟基苯甲酸、藜芦酸、阿魏酸、丁香酸、肉桂酸、芥子酸和绿原酸9种酚酸物质的高效液相色谱法检测及姜黄来源对其酚酸物质含量的影响。采用APOLLO C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相A相为0.25%乙酸水溶液,流动相B相为85%甲醇水溶液,梯度洗脱,流速0.9m L/min,VWD检测波长283 nm,柱温30℃。检测方法显示,9种酚酸物质的检测线性范围为0.05~200 mg/L;检出限(S/N=3)在0.003~0.07 mg/kg,在加标浓度2.0~100.0 mg/kg条件下,加标回收率(N=6)在91.01%~103.09%,检测方法灵敏有效。检测结果表明,不同来源姜黄提取物得到的姜黄提取物中水杨酸、间羟基苯甲酸、对羟基苯甲酸、藜芦酸、阿魏酸、丁香酸、肉桂酸、芥子酸和绿原酸9种姜黄提取物酚酸物质总含量以四川乐山产姜黄提取物最高。
Comparison of different sources of turmeric extract of salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, veratric acid, ferulic acid, syringic acid, cinnamic acid, Chromatographic detection and turmeric source on phenolic acids content. The mobile phase A was 0.25% acetic acid aqueous solution and the mobile phase B was 85% aqueous methanol. The gradient elution was performed on an APOLLO C 18 column (150 mm × 4.6 mm, 5 μm) with a flow rate of 0.9 m L / min. The VWD Detection wavelength 283 nm, column temperature 30 ℃. The detection methods showed that the linear range of detection of nine phenolic acids was 0.05-200 mg / L, the detection limit (S / N = 3) was 0.003-0.07 mg / kg, and the spiked concentration was 2.0-100.0 mg / kg Under the optimal conditions, the spiked recoveries (N = 6) ranged from 91.01% to 103.09%. The detection method was sensitive and effective. The test results showed that salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, veratric acid, ferulic acid, syringic acid, cinnamic acid, erucic acid and chlorogenic acid in the turmeric extract obtained from different origins of turmeric extract Curcuma longa total content of phenolic acids produced in Leshan, Sichuan, the highest turmeric extract.