Describes the general design of Microbiosensors for determination of glucose in blood sample for medical purpose as an important branch of medical analysis instrument discusses the fabrication of microbiosensors. By the technology of microfabrication, self assembled monolayers (SAMs) and enzyme immobilization, stresses their properties, such as improvement in system efficiency, shorter analysis time reduction of reagent waste and reduction of device sizes, which makes them suitable for bed side monitor of emergency patients. Describes the general design of Microbiosensors for determination of glucose in blood sample for medical purpose as an important branch of medical analysis instrument to the fabrication of microbiosensors. By the technology of microfabrication, self assembled monolayers (SAMs) and enzyme immobilization, stresses their properties, such as improvement in system efficiency, shorter analysis time reduction of reagent waste and reduction of device sizes, which makes them suitable for bed side monitor of emergency patients.