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背景与目的骨转移占晚期肺癌的50%-70%。本研究以体外侵袭、迁移能力不同的肺腺癌细胞系A549、H1299、SPC-A-1、XL-2为基础建立肺腺癌骨转移裸小鼠模型,MicroCT观察骨转移情况。方法将50只6 w-8 w龄裸小鼠随机平均分为5组,4个实验组左心室分别注射相应四种细胞悬液(0.2 mL/只);对照组左心室注射等量生理盐水。注射后第二周起定期对各组小鼠进行MicroCT扫描,当小鼠明显消瘦时此组观察结束,结束前行骨组织病理学检查;对各实验组出现的骨转移部位按中轴骨和四肢骨归类,比较这两种部位之间的转移率;根据各组出现骨转移所用平均时间、骨转移率,对各细胞系骨转移能力进行统计分析。结果经MicroCT、病理学检查确定,各实验组出现不同骨转移率,对照组小鼠无骨转移现象;各实验组中轴骨转移率均明显高于四肢骨,这与临床上肺癌骨转移规律一致,模型建立成功。各实验组间发生骨转移的小鼠数目及出现转移所用平均时间无明显差异。结论MicroCT能清晰地检测到骨质破坏,利于骨转移情况的判断;我们成功建立了肺腺癌骨转移模型,为以后探索出新的肺腺癌乃至肺癌骨转移临床预防和治疗方案提供基础;4种肺腺癌细胞系体外侵袭、迁移能力强弱不等,但体内骨转移能力没有明显差异,其原因还有待进一步的探索。
Background and Objective Bone metastasis accounts for 50% -70% of advanced lung cancer. In this study, nude mice models of lung adenocarcinoma with bone metastasis were established on the basis of lung adenocarcinoma cell lines A549, H1299, SPC-A-1 and XL-2 with different invasion and migration capacities. MicroCT was used to observe the bone metastasis. Methods Fifty (50) w 6-week-old nude mice were randomly divided into five groups randomly. The left ventricle of four experimental groups were injected with four kinds of cell suspensions (0.2 mL / each) respectively. In the control group, . The second week after injection, the mice in each group were subjected to MicroCT scan regularly. When the mice were obviously wasting, the observation of this group was over and the histopathological examination was completed. The bone metastases occurred in each experimental group according to the axis of the central axis According to the mean time of bone metastasis and the bone metastasis rate in each group, statistical analysis was made on the bone metastasis ability of each cell line. Results MicroCT and pathological examinations showed that there were different rates of bone metastasis in each experimental group and no bone metastasis in control group. Axillary bone metastasis rate in each experimental group was significantly higher than that in limbs, which was associated with clinical bone metastasis of lung cancer Consistent, the model is established successfully. There was no significant difference in the number of mice with bone metastases and the mean time to metastasis between experimental groups. Conclusion MicroCT can clearly detect the bone destruction and facilitate the judgment of bone metastasis. We have successfully established a model of bone metastasis of lung adenocarcinoma, which will provide the basis for clinical prevention and treatment of new lung adenocarcinoma and even bone metastasis of lung cancer in the future. The four kinds of lung adenocarcinoma cell lines in vitro invasion and migration of varying degrees of strength, but there was no significant difference in bone metastasis, the reason remains to be further explored.