背景:乌龙丹改善多梗死性痴呆(multi-infarctdementia,MID)学习记忆功能障碍,其作用机制是否与部分氨基酸含量降低有关?目的:探讨乌龙丹对多梗死性痴呆模型大鼠学习记忆功能和脑组织20种氨基酸含量变化的影响。设计:随机对照实验研究。地点和材料:实验地点:解放军第一军医大学中医系实验室和附属珠江医院神经内科实验室。取学习记忆合格大鼠(n=40),雌雄各半,体质量(280±30)g,随机分为空白对照组(n=8)、乌龙丹高剂量组(n=8)、低剂量组(n=8)、喜得镇组(n=8)、模型组(n=8)。干预:分离大鼠一侧颈内动脉,推注1∶200比例的干血悬液0.8mL造模。氨基酸测定用反相高效液相色谱法,乌龙丹的制剂采用水煮醇提法,灌胃给药。主要观察指标:各组大鼠Y型迷宫学习测试和脑组织20种氨基酸含量的变化。结果:模型组大鼠躲避电击错误次数最多;乌龙丹给药组的错误次数明显减少(P<0.05);用药组和对照组自身对照,造模前后大鼠的学习记忆水平有显著性差异(P<0.05);大鼠造模后,脑组织中兴奋性氨基酸天冬氨酸、谷氨酸的含量明显降低;而乌龙丹高剂量组的谷氨酸含量与模型组有显著性差异(F=3.672,P<0.05);大鼠造模后脑组织中抑制性氨基酸γ-氨基丁酸、组氨酸、牛氨酸含量明显降低,乌龙丹高剂量组γ-氨基丁酸、牛氨酸、甘氨酸,低剂量组 BACKGROUND: Wulongdan improves learning and memory dysfunction in patients with multi-infarct dementia (MID), and its mechanism of action is related to the decrease of some amino acid content. Objective: To study the effect of Wulongdan on learning and memory in rats with multi-infarct dementia (MDE). And the effect of changes in the content of 20 amino acids in brain tissue. Design: Randomized controlled experimental study. Location and Materials: The experimental site: Laboratory of Traditional Chinese Medicine of the First Military Medical University of PLA and Laboratory of Neurology of Zhujiang Hospital Affiliated to the PLA. Rats with learning and memory ability (n=40), half male and half female, body weight (280±30) g were randomly divided into blank control group (n=8), Wulongdan high-dose group (n=8), and low Dose group (n=8), Xideng group (n=8), model group (n=8). Intervention: The rat’s internal carotid artery was isolated and injected with 0.8 mL of dry blood suspension at a 1:200 ratio. Amino acids were measured by reversed-phase high-performance liquid chromatography. The preparations of Wulongdan were administered by boiling water and intragastrically. MAIN OUTCOME MEASURES: The Y-maze learning test and the changes of 20 kinds of amino acid content in brain tissue of each group of rats. RESULTS: The rats in the model group had the most mistakes in avoiding electric shocks; the number of errors in the Wulongdan group was significantly reduced (P<0.05); the self-control group and the control group had significant differences in the learning and memory levels between the rats before and after the modeling. (P<0.05); After the rats were modeled, the content of aspartic acid and glutamate in the brain tissue was significantly decreased; while the glutamate content in the high dose group of Wulongdan was significantly different from the model group. (F=3.672, P<0.05); The content of inhibitory amino acids γ-aminobutyric acid, histidine, and taurine in brain tissue was significantly decreased in rats after modeling, and γ-aminobutyric acid and cattle in high dose group in Wulongdan Glycine, glycine, low dose group