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目的观察木豆叶提取物对抗维甲酸导致大鼠骨质疏松的作用。方法取SD大鼠60只,雌雄各半,按体质量随机分为5组,即正常组,模型组,仙灵骨葆阳性药组(300 mg·kg~(-1)),木豆叶提取物高、低剂量组(8,0.8 g·kg~(-1))。除正常组外,每组大鼠均上午灌胃维甲酸14 d,造模同时各组分别给予相应剂量的药物进行干预,连续给药4周。观察木豆叶提取物对大鼠体质量、脏器指数以及骨钙、磷、骨骼灰化率和骨组织形态学的影响。结果与模型组比较,木豆叶提取物低剂量组能显著提高肾上腺和前列腺的指数(P<0.05),高剂量组能减少脾脏指数(P<0.05);木豆提取物高、低剂量组对股骨和椎骨骨钙、磷含量及骨骼灰化率都有显著提高作用(P<0.05,P<0.001);病理形态学指标结果显示,模型组动物皮质骨面积百分比、骨小梁平均厚度、骨小梁面积、骨小梁体积、成骨细胞数均较正常组明显减少(P<0.001),而破骨细胞数明显增多(P<0.001)。木豆叶提取物低剂量组能明显对抗上述指标中除成骨细胞数的变化(P<0.001),高剂量组对除骨小梁厚度和成骨细胞数之外的上述指标变化有逆转作用(P<0.05,P<0.01,P<0.001)。结论木豆叶提取物能较好地改善维甲酸造成的大鼠骨质疏松。
Objective To observe the effect of extract of pigeons leaf on the osteoporosis induced by retinoic acid in rats. Methods Sixty male Sprague-Dawley rats were randomly divided into 5 groups according to their body weight, namely normal group, model group, Xianling Gubao Decoction (300 mg · kg -1) The extract of high and low dose group (8, 0.8 g · kg -1). Except for the normal group, rats in each group were given retinoic acid for 14 days in the morning, while the rats in each group were given the corresponding doses of drugs for intervention for 4 weeks. To observe the effects of extract of Magnolia biondii on body weight, viscera index, calcium, phosphorus, bony ashing rate and histomorphology in rats. Results Compared with the model group, the low dose group of GAO could significantly increase the index of adrenal and prostate (P <0.05), the high dose group could reduce the index of spleen (P <0.05) (P <0.05, P <0.001). The results of pathomorphology showed that the percentage of cortical bone area, the average thickness of trabecular bone, Trabecular area, trabecular volume and osteoblast number were significantly decreased (P <0.001), while the number of osteoclasts was significantly increased (P <0.001). The low dose group of M. esculentum extract could obviously resist the change of the number of osteoblasts (P <0.001), and the high dose group reversed the change of trabecular bone thickness and the number of osteoblasts (P <0.05, P <0.01, P <0.001). Conclusion GAO extract can effectively improve rat osteoporosis caused by RA.