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目的:观察N-糖基化对突触细胞黏附分子(Syn-CAM)调节突触形成作用的影响。方法:制备SynCAMN-糖链定点突变质粒和正常SynCAM表达质粒转染海马神经元细胞,膜片钳试验观察突触中的转染质粒的海马神经元作为突触后部分其动作电位的潜伏期和频率。结果:突变SynCAM质粒组海马神经元动作电位潜伏期为(180.9±3.5)ms(n=14),频率为(10.2±0.7)/s(n=14)。正常SynCAM质粒组海马神经元动作电位潜伏期为(174.7±4.8)(n=14),动作电位频率为(10.0±0.8)/s(n=14),差异无统计学意义。结论:有无N-糖链缺失的SynCAM对突触功能的影响无明显差别,下游信号蛋白可能影响SynCAM调节功能。
Objective: To observe the effect of N-glycosylation on the synaptic function of Syn-CAM. Methods: SynCAMN-sugar chain site-directed mutagenesis plasmid and normal SynCAM expression plasmid were transfected into hippocampal neurons. Patch clamp test was used to observe the latency and frequency of the action potential of synapse-transfected hippocampal neurons as the postsynaptic part . Results: The latency of action potential of hippocampal neurons in mutant SynCAM plasmid group was (180.9 ± 3.5) ms (n = 14) and frequency was (10.2 ± 0.7) / s (n = 14). SynCAM plasmid in normal hippocampal neurons had an incubation period of (174.7 ± 4.8) (n = 14) and an action potential of (10.0 ± 0.8) / s (n = 14), with no significant difference. Conclusion: SynCAM with or without N-linked glycans has no significant effect on synaptic function, and downstream signaling proteins may affect SynCAM regulatory function.