目的分析ob/ob肥胖小鼠脂肪肝中脂质代谢相关基因的表达特征。方法 18周龄雄性ob/ob及其同窝对照小鼠各4只,禁食16h后处死。检测小鼠体质量、肝脏湿质量/体质量、肝脏三酰甘油(TG)水平;用H-E和油红O染色观察肝脏的形态结构和脂质沉积情况;用实时荧光定量PCR方法检测肝脏脂质代谢相关基因的mRNA表达情况。结果 Ob/ob小鼠体质量、肝脏湿质量/体质量和肝脏组织TG水平均高于对照小鼠(P<0.05),H-E和油红O染色显示ob/ob小鼠发生肝脏脂质沉积;肝脏脂肪酸转位酶(CD36)、脂肪酸结合蛋白1(FABP1)、脂肪酸合酶(FASN)、乙酰辅酶A羧化酶1(ACC1)、极长链脂肪酸延长酶6(ELOVL6)和硬脂酰辅酶A去饱和酶1(SCD1)等mRNA表达水平在ob/ob小鼠肝脏均高于对照小鼠(P<0.05),而过氧化物酶体增殖剂激活受体α(PPARα)、软脂酸辅酶A氧化酶1(ACOX1)、载脂蛋白B(ApoB)和微粒体TG转移蛋白(MTP)mRNA在两组小鼠肝脏中的mRNA表达水平差异无统计学意义。结论 Ob/ob小鼠肝脏的脂肪酸摄取和从头合成相关基因的mRNA表达水平升高,而脂肪酸氧化及脂质向肝外转运的相关基因的mRNA表达水平无明显改变。 Objective To analyze the expression characteristics of lipid metabolism related genes in fatty liver of ob / ob mice. METHODS: Four male ob / ob mice and their littermate control mice, 18 weeks old, were sacrificed after 16 hours of fasting. The body weight, liver wet weight / body weight, and liver triglyceride (TG) level were measured. The morphology and lipid deposition of the liver were observed by HE and oil red O staining. The liver lipid Metabolic related gene mRNA expression. Results Ob / ob mice body weight, liver wet weight / body weight and liver tissue TG level were higher than those in control mice (P <0.05). HE and O-R staining showed obesity in ob / ob mice. (CD36), FABP1, FASN, ACC1, ELOVL6 and stearoyl-CoA A desaturase 1 (SCD1) mRNA expression in the ob / ob mouse liver were higher than the control mice (P <0.05), while the peroxisome proliferator-activated receptor alpha (PPARα), palmitate There was no significant difference in the mRNA expression of ACOX1, ApoB and MTP mRNA between the two groups of mice. Conclusion The mRNA expression of fatty acid uptake and de novo gene synthesis in ob / ob mouse liver increased, while the mRNA expression of fatty acid oxidation and lipid extracellular transport did not change significantly.