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利用酿酒酵母作为生物催化剂合成L-苯基乙酰基甲醇(L-Phenylacetylcarbiol,L-PAC)的过程中,通过加入有机溶剂(辛醇)形成两相体系,可改善底物和产物的溶解度,以减少对细胞内丙酮酸脱羧酶(PDC)的强烈抑制和钝化作用,从而促进L-PAC产量的提高。实验结果表明,两相体系合成L-PAC的最佳条件为活化60 min,苯甲醛的起始浓度940 mmol/L,丙酮酸钠的起始浓度460 mmol/L,葡萄糖的含量8%,水醇两相体积比为3∶1,且加入一定量的甘油时,L-PAC产量可达在醇相中24.1 g/L和水相中3.7 g/L,比单一水相体系高出1.8倍。
In the synthesis of L-Phenylacetylcarbiol (L-PAC) using Saccharomyces cerevisiae as a biocatalyst, the solubility of the substrate and the product can be improved by the addition of an organic solvent (octanol) to form a two-phase system Reduce the intracellular pyruvate decarboxylase (PDC) strong inhibition and passivation, thereby promoting the L-PAC yield. The experimental results showed that the optimum conditions for synthesizing L-PAC in two-phase system were activation 60 min, initial concentration of benzaldehyde 940 mmol / L, initial concentration of sodium pyruvate 460 mmol / L, glucose 8%, water Alcohol volume ratio of 3:1, and adding a certain amount of glycerol, L-PAC production up to 24.1 g / L in the alcohol phase and 3.7 g / L in the aqueous phase, 1.8 times higher than the single aqueous system .