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试验以油幼胚为材料,研究外植体大小、低温处理、基本培养基类型、碳源、培养基中的2,4-D浓度、AgNO3的添加与否对胚性愈伤组织诱导的影响,结果表明,长轴为5~7.9 mm低温处理18 h的幼胚,诱导胚性愈伤组织的效果最好;使用山梨醇(浓度为3%)为碳源、含氮量低的WPM培养基,胚性愈伤组织的诱导效果优于蔗糖为碳源、含氮量高的MS培养基;幼胚胚性愈伤组织诱导的最适2,4-D浓度为2.0 mg/L,培养基中附加5.0 mg/L AgNO3有助于提高胚性愈伤组织的诱导率。
The effects of AgNO3 on the induction of embryogenic callus were studied by using the oil-baby embryo as material, and the explants size, low-temperature treatment, basic medium type, carbon source, 2,4-D concentration in medium, The results showed that embryo callus was best induced by embryos with long axis of 5 ~ 7.9 mm and treated for 18 h at low temperature. WPM culture with sorbitol (3% concentration) as carbon source and low nitrogen content The induction effect of embryogenic callus was better than MS medium with sucrose as carbon source and high nitrogen content. The optimum 2,4-D concentration induced by immature embryo callus was 2.0 mg / L, Addition of 5.0 mg / L AgNO3 to the base helps to improve the induction rate of embryogenic callus.