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本文报道了一种用13只实验动物进行研究和测定LD_(50)的新方法。作者评价了目前各种LD_(50)测定及其统计方法,指出这些方法都要求相当数目的实验动物并用增加动物的数目来提高LD_(50)值的准确性。但非常精确的LD_(50)值对同类生物测定的重现性差,加上从动物外推到人的困难,故其精确计算意义不大。同时,急性中毒表现、急性毒性的靶器官、损害的可逆性等资料,用少数动物进行细致的观察就可获得。因此,用较少的动物来进行急性毒性研究和LD_(50)值的测定是可行的。
In this paper, we report a new method for the study and determination of LD_ (50) in 13 experimental animals. The authors evaluated various current LD 50 assays and their statistical methods, pointing out that these methods all require a significant number of experimental animals and increasing the number of animals to increase the accuracy of LD 50 values. However, the very accurate LD 50 value has poor reproducibility for similar bioassays and extrapolation from animal to human, so its exact calculation is of little value. At the same time, acute poisoning, acute toxicity of target organs, damage reversibility and other data, with a few animals can be carefully observed. Therefore, the use of fewer animals for acute toxicity studies and determination of LD_ (50) values is feasible.