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采用整体化色谱柱,对复配制剂14.5%胺苯·草除·精氟吡甲可湿性粉剂中的三种有效成分胺苯磺隆、草除灵乙酯、精氟吡甲禾灵进行了分离测定。在整体化色谱柱Chromolith O R Performance RP-18e上以乙腈-水溶液为流动相作梯度洗脱,检测波长为254nm的条件下,在15min内可达到基线分离。在0.02mg/mL～0.4mg/mL时三种有效成分的浓度与峰面积呈良好的线性关系,加标回收率在98.6%～100.8%,相对标准偏差(RSD)(n=6)在0.35%～0.66%,应用该方法可在一次进样中同时完成三种有效成分的定量分析,操作简便,快速准确。
Using the integrated column, the active ingredient of the mixture of 14.5% of the amine benzene · grass in addition to fine fluoride pirfenidone hydrochloride, triamcinolone, Isolated assay. The chromatographic column Chromolith O R Performance RP-18e was eluted with a mobile phase of acetonitrile-water as a mobile phase at a detection wavelength of 254 nm. Baseline separation was achieved within 15 min. The linear range of the three active ingredients was 0.02mg / mL ~ 0.4mg / mL, the recoveries were in the range of 98.6% ~ 100.8%, and the relative standard deviations (RSDs) were 0.35 % ~ 0.66%. By this method, the quantitative analysis of three active ingredients can be completed in one injection, which is easy to operate and fast and accurate.