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输卵管炎最常见的病因是沙眼衣原体和淋球菌感染。对衣原体性输卵管炎流行病学性质和发病机理缺乏了解的主要原因是沙眼衣原体检测的困难。培养沙跟衣原体费时且技术难度大,更新的衣原体检测技术如单克隆抗体或衣原体特异性DNA探针的敏感性并不比培养好,而且与其它细菌有交叉反应,可出现假阳性结果。本研究采用的PCR技术克服了以上不足。 实验应用的DNA引物选在编码衣原体主要外层膜蛋白的基因区,扩增片段144bp,内含1个EcoR Ⅰ酶切位点。PCR循环参数为95℃,1min;55℃,1min;72℃,2min。酶切时产生103bp产物。研究对象为15例经腹腔镜确诊的急性输卵管炎
The most common causes of salpingitis are Chlamydia trachomatis and Neisseria gonorrhoeae infection. Chlamydia salpingitis on the epidemiological nature and lack of understanding of the pathogenesis of the main reason for the detection of Chlamydia trachomatis difficulties. Chlamydia trachomatis is time-consuming and technically challenging. Updated chlamydial detection techniques such as monoclonal antibodies or chlamydia-specific DNA probes are no more sensitive than cultivated and cross-reactive with other bacteria, giving false positive results. The PCR technique used in this study overcomes the above shortcomings. The DNA primers used in the experiment were selected in the region encoding the major outer membrane protein of Chlamydia, and the amplified fragment was 144bp containing one EcoRI restriction site. PCR cycling parameters 95 ℃, 1min; 55 ℃, 1min; 72 ℃, 2min. Upon cleavage, a 103 bp product was produced. The object of study was 15 cases of acute salpingitis diagnosed by laparoscopy