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The single spores were isolated from chlamydospores of Ustilaginoidea virens with three different maturities by PSA. The isolated single spores were cultured on different media at different temperatures under natural light for inducing conidia to explore the optimum isolation technique of single spore and optimum culture condition of conidia. The results showed that the successful isolating rate of single spore from yellow rice false smut balls reached 90. 00% . The sporulation quantities of isolated single spores cultured on PSD and PDB media at 22 - 29 ℃ ( variable temperature under natural light) or 28 ℃ ( constant temperature under dark condition) for 12 d were up to 6. 3 × 107 and 1. 1 × 106 spore / mL,respectively. PSA was the most effective method to isolate single spores from yellow rice false smut balls of U. virens. The optimum conidia culture condition included PSD or PDB medium,22 - 29 ℃ or 28 ℃ ,natural light and vibration culture.
The single spores were isolated from chlamydospores of Ustilaginoidea virens with three different maturities by PSA. The isolated single spores were cultured on different media at different temperatures under natural light for inducing conidia to explore the optimum isolation technique of single spore and optimum culture condition of conidia . The results showed that the successful isolating rate of single spore from yellow rice false smut balls reached 90. 00%. The sporulation quantities of isolated single spores cultured on PSD and PDB media at 22 - 29 ° C (variable temperature under natural light) or 28 ° C (constant temperature under dark condition) for 12 d were up to 6. 3 × 107 and 1. 1 × 106 spore / mL, respectively. PSA was the most effective method to isolate single spores from yellow rice false smut balls of U The optimum conidia culture condition included PSD or PDB medium, 22 - 29 ° C or 28 ° C, natural light and vibration culture.