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目的探讨黄芪甲苷对异丙肾上腺素诱导HL-1心肌细胞活性及维生素D受体mRNA相对表达量的影响。方法用异丙肾上腺素(isoproterenol,ISO)10μmol·L~(-1)诱导HL-1心肌细胞损伤的模型,随机分为5组:正常组,模型组,模型组+黄芪甲苷(3μmol·L~(-1))(即黄芪甲苷低剂量组),模型组+黄芪甲苷(10μmol·L~(-1))(即黄芪甲苷中剂量组),模型组+黄芪甲苷(30μmol·L~(-1))(即黄芪甲苷高剂量组)。运用MTT法检测心肌细胞的存活率。RT-PCR检测维生素D受体mRNA的相对表达量。结果 ISO处理HL-1心肌细胞,不同浓度的黄芪甲苷可提高心肌细胞的活性,且黄芪甲苷的浓度在30μmol·L~(-1)时,保护作用最好。RT-PCR检测结果显示,不同剂量的黄芪甲苷VDR基因相对表达量与模型组相比,其表达明显下调(P<0.05)。结论黄芪甲苷对HL-1心肌细胞损伤具有保护作用,其机制可能通过维生素D轴调节VDR基因表达水平有关。
Objective To investigate the effects of Astragaloside IV on isoproterenol-induced HL-1 cardiomyocyte activity and the relative expression of vitamin D receptor mRNA. Methods The model of HL-1 cardiomyocyte injury induced by isoproterenol (ISO) 10μmol·L -1 was randomly divided into 5 groups: normal group, model group, model group + Astragaloside (3μmol · (-1) Astragaloside group (low dose Astragaloside group), model group + Astragaloside (10μmol·L -1) (Astragaloside medium dose group), Astragaloside group 30μmol·L -1) (that is, high-dose Astragaloside group). The survival rate of cardiomyocytes was detected by MTT method. RT-PCR detection of vitamin D receptor mRNA relative expression. Results ISO treatment of HL-1 cardiomyocytes, different concentrations of astragaloside can improve myocardial cell activity, and the concentration of Astragaloside at 30μmol·L -1, the best protective effect. The results of RT-PCR showed that the relative expression levels of VDR gene at different doses of Astragaloside IV were significantly down-regulated compared with the model group (P <0.05). Conclusion Astragaloside has a protective effect on the injury of HL-1 cardiomyocytes, which may be related to the regulation of VDR gene expression by vitamin D axis.