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用两套具有不同时间分辨率和激发方式的测量系统对菌紫质(BR)光循环动力学进行了泵浦一探测研究。一套是单次脉冲氙灯闪光诱导样品;另一套采用重复调Q倍频YAG激光器周期性激发样品。检测光使用He-Ne激光器633um红光。用雪崩光电二极管和500MHz数字示波器进行信号检测与波形处理。实验结果表明,菌紫质光循环周期<15ms。中间体K→L的时间常数为2μs,L→M的时间常数为50μs,在中间体K之前发现了一个可疑的成份。据此给出了菌紫质光循环在纳秒到毫秒时域的模型。
The photocycle dynamics of the bacteriorhodopsin (BR) was pumped and probed with two sets of measurement systems with different time resolution and excitation modes. One is a single-shot xenon flash flash induction sample; the other is a periodic Q-frequency doubled QAG YAG laser to periodically excite the sample. Detection of light using He-Ne laser 633um red. Use avalanche photodiodes and 500MHz digital oscilloscopes for signal detection and waveform processing. Experimental results show that the bacteriorhodopsin photoperiod cycle <15ms. The time constant of intermediate K → L is 2μs, the time constant of L → M is 50μs, and a suspect component is found before intermediate K. Based on this, the model of bacterial purple light cycle in nanosecond to millisecond time domain is given.