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目的 :研究口腔鳞癌与人乳头瘤病毒 (HPV)、疱疹病毒I型 (HSV -I)和人巨细胞病毒 (HCMV)的关系。方法 :采用斑点杂交和PCR技术检测 32例口腔鳞癌、14例口腔白斑和 10例正常口腔粘膜中HPV1 6 、HSV -I及HCMVDNA。结果 :在正常口腔粘膜、白斑及口腔鳞癌中HPV1 6 、HSV -I及HCMVDNA感染率分别为 0 %、35 7%、5 0 0 % ,40 0 %、5 0 0 %、43 3%和 0 %、14 3%、2 8 1% ,口腔鳞癌及白斑组织中HPV1 6 -DNA的检出率均高于正常口腔 ,且差别具有显著性 (p <0 0 5 ) ;但HSV -I和HCMVDNA在口腔疾患中的检出率与正常比较无显著差别 (p >0 0 5 )。结论 :HPV1 6 感染与口腔鳞癌的发生相关 ;HSV -I和HCMV可能参予口腔鳞癌的发生及发展 ,并且与HPV1 6 有协同致癌的作用
Objective: To study the relationship between oral squamous cell carcinoma and human papillomavirus (HPV), herpesvirus type 1 (HSV-I) and human cytomegalovirus (HCMV). Methods: HPV16, HSV-I and HCMVDNA were detected in 32 cases of oral squamous cell carcinoma, 14 cases of oral leukoplakia and 10 cases of normal oral mucosa by dot blot hybridization and PCR. Results: The infection rates of HPV16, HSV-I and HCMVDNA in normal oral mucosa, leukoplakia and oral squamous cell carcinoma were 0%, 35 7%, 500%, 40 0%, 500%, 43 3% and The positive rate of HPV16-DNA in oral squamous cell carcinoma and leukoplakia was higher than that in normal oral cavity (p <0.05), but the positive rates of HSV-I And HCMVDNA in oral disease detection rate compared with the normal no significant difference (p> 0.05). CONCLUSIONS: HPV1 6 infection is associated with the occurrence of oral squamous cell carcinoma. HSV-I and HCMV may participate in the carcinogenesis and development of oral squamous cell carcinoma and have synergistic carcinogenesis with HPV16