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本研究利用中棉所36和海1配制杂交组合,并用中棉所36为轮回亲本构建高代回交群体。利用3223对SSR引物筛选亲本和F1,筛选到294对含有海1显性带的引物,占筛选引物总数的9.12%。最终对其中的267对引物进行了群体扩增,获得277个SSR标记差异位点。其中,217个标记位点连锁,构建44个连锁群,平均每个连锁群包含4.93个标记位点。图谱覆盖1908.67cM,占棉花基因组的42.89%,平均每个连锁群覆盖43.38cM,标记间平均相距8.80cM。利用陆海杂种BC1F1、BC2F1和BC1S1的表型数据,通过复合区间作图,共检测到9个QTL,解释表型变异6.90%￣19.17%。其中3个增效基因来自海岛棉亲本海1,6个增效基因来自陆地棉亲本中棉所36。此外,控制衣分的2个QTL在3个世代都能够检测到,分别解释6.90%和19.17%的表型变异,效应稳定,为进一步分子标记辅助选择奠定了基础。
In this study, Zhongmiansuo 36 and Hai 1 were used to construct a cross combination and a high-generation backcross population was constructed using the Zhongmiansuo36 as a recurrent parent. Using 3223 pairs of SSR primers to screen parents and F1, 294 primers were screened, which accounted for 9.12% of the total number of primers. Finally, 267 pairs of primers were amplified and 277 SSR markers were obtained. Among them, 217 marker loci were linked to construct 44 linkage groups, with an average of 4.93 marker loci per linkage group. The map covered 1908.67cM, accounting for 42.89% of the total genome of cotton. The average coverage of each linkage group was 43.38cM, and the average distance between markers was 8.80cM. Nine phenotypic QTLs were detected by composite interval mapping based on the phenotypic data of BC1F1, BC2F1 and BC1S1, which explained the phenotypic variation 6.90% ~ 19.17%. Among them, three of the three synergistic genes came from island cotton parents and six of them were from cotton plants of upland cotton. In addition, two QTLs for control of lint percentage were detected in three generations, accounting for 6.90% and 19.17% of phenotypic variation, respectively. The effect was stable and laid the foundation for further molecular marker-assisted selection.