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目的 了解急性淋巴细胞白血病细胞核基质蛋白与正常相应细胞的差异、化疗前后急性淋巴细胞核基质蛋白的影响、温度对核基质蛋白的影响以及部分核基质蛋白的等电点。方法 用高盐法提取核基质蛋白,SDS—PAGE、2—D电泳分析核基质蛋白的变化。结果 在分子量为60KD、65KD 75KD处出现了正常相应细胞所没有的核基质蛋白,急性淋巴细胞白血病化疗后分子量为65KD、75KD的核基质蛋白消失,60KD、90KD处核基质蛋白减少,温度在42℃、30min时急性淋巴细胞白血病细胞核基质蛋白受破坏。结论 急性淋巴细胞白血病与正常相应细胞相比有组分的改变,核基质蛋白是化疗药物的作用位点,核基质蛋白对温度敏感,分子量为60KD、65KD、75KD的核基质蛋白等电点分别是6.5,6.2,6.0。
Objective To investigate the difference of nuclear matrix proteins and normal corresponding cells between acute lymphoblastic leukemia and acute lymphoblastic leukemia cells before and after chemotherapy, the effect of temperature on nuclear matrix proteins and the isoelectric point of some nuclear matrix proteins. Methods The nuclear matrix proteins were extracted by high salt method. The changes of nuclear matrix proteins were analyzed by SDS-PAGE and 2-D electrophoresis. Results At the molecular weight of 60KD and 65KD at 75KD, nuclear matrix proteins were found in normal corresponding cells. The molecular weight of 65KD and 75KD nuclear matrix protein disappeared after chemotherapy. The nuclear matrix protein of 60KD and 90KD decreased at the temperature of 42 ℃, 30min acute lymphoblastic leukemia cell nuclear matrix protein destruction. CONCLUSIONS: Acute lymphoblastic leukemia has a component change compared with normal corresponding cells. Nuclear matrix protein is the site of chemotherapeutic drugs. Nuclear matrix proteins are sensitive to temperature. The isoelectric points of nuclear matrix proteins with molecular weights of 60KD, 65KD and 75KD are respectively Is 6.5, 6.2, 6.0.