论文部分内容阅读
目的探讨气道内应用白细胞介素 12 (IL- 12 )对抗原诱导的过敏性反应的影响。方法 C5 7BL/ 6小鼠经 OVA免疫建立哮喘模型 ,在主动免疫及抗原激发阶段气道内应用 IL- 12 ,观察肺泡灌洗液细胞成份、肺部淋巴细胞产生细胞因子、外周血 Ig E水平变化。结果 1致敏阶段应用 IL- 12可明显抑制嗜酸性粒细胞浸润、肺淋巴细胞对 IL- 5的分泌以及血浆总 Ig E和抗原特异性 Ig E的水平 ;2激发阶段应用 IL- 12可明显抑制嗜酸性粒细胞的浸润 ,抑制肺淋巴细胞产生 IL- 4、IL- 5 ,增加其产生 IFN- γ,但对抗原特异性 Ig E无明显影响 ;3如致敏和激发阶段均应用 IL- 12 ,则明显抑制肺淋巴细胞产生 IL- 4、IL- 5 ,增强 IFN- γ产生 ,抑制气道内嗜酸性粒细胞的浸润及血浆 Ig E的升高。结论气道应用 IL- 12对抗原诱导的气道过敏性炎症有明显调节作用 ,且与应用时机有关 ,为 IL- 12治疗哮喘提供依据
Objective To investigate the effect of intratracheal administration of interleukin-12 (IL-12) on antigen-induced allergic reactions. Methods The asthmatic model was established by OVA immunization in C5 7BL / 6 mice. IL-12 was used in the airway during active immunization and antigen challenge, and the changes of cellular components in lung lavage fluid, cytokines in lung lymphocytes and IgE in peripheral blood were observed . Results 1 IL-12 sensitized phase can significantly inhibit eosinophil infiltration, lung lymphocytes on IL-5 secretion and plasma total Ig E and antigen-specific Ig E levels; 2 excitation phase IL-12 can be significantly Inhibit the infiltration of eosinophils, inhibit the production of IL-4 and IL-5 in lung lymphocytes, and increase their production of IFN-γ, but have no obvious effect on antigen-specific IgE; 3, such as IL- 12 , Significantly inhibited the production of IL-4 and IL-5 in lung lymphocytes, enhanced the production of IFN-γ, inhibited the infiltration of eosinophils in airway and the increase of plasma IgE. Conclusion Airway IL-12 can obviously regulate airway allergic inflammation induced by antigens, and it is related to the timing of application, providing a basis for the treatment of asthma with IL-12