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目的:探讨神经纤毛蛋白(NRP-1)在子宫内膜癌细胞系中的表达及17β雌二醇(17β-E2)对其表达的调控。方法:采用RT-PCR法及Western blot法检测NRP-1在子宫内膜癌HEC-1A及Ishikawa细胞系中的表达;荧光定量PCR法检测17β-E2对HEC-1A及Ishikawa细胞系中NRP-1 mRNA表达的调控;Western blot法检测Ishikawa细胞中NRP-1表达的变化。结果:HEC-1A及Ishikawa细胞中NRP-1 mRNA及蛋白水平均为阳性表达。17β-E2对Ishikawa细胞系中NRP-1 mRNA表达的影响显著强于HEC-1A,且17β-E2浓度为2×10-7mol/L,作用时间48h时对NRP-1 mRNA表达的促进作用最明显。17β-E2对Ishikawa细胞中NRP-1蛋白表达呈时间依赖性。结论:NRP-1表达于高表达雌激素受体(ER)细胞系Ishikawa及低表达ER细胞系HEC-1A中,且2×10-7mol/l E2作用48h能明显促进Ishikawa细胞系中NRP-1的表达,提示NRP-1可能与子宫内膜癌的发生发展密切相关。
Objective: To investigate the expression of NRP-1 in endometrial carcinoma cell lines and its regulation by 17β-estradiol (17β-E2). Methods: The expression of NRP-1 in HEC-1A and Ishikawa cell lines was detected by RT-PCR and Western blot. The expression of NRP-1 in HEC-1A and Ishikawa cell lines was detected by real-time quantitative PCR. 1 mRNA expression; Western blot method to detect Ishikawa cells NRP-1 expression changes. Results: The mRNA and protein levels of NRP-1 in HEC-1A and Ishikawa cells were all positive. The effect of 17β-E2 on NRP-1 mRNA expression in Ishikawa cell line was significantly stronger than that of HEC-1A, and the effect of 17β-E2 at 2 × 10-7mol / L and 48h obvious. 17β-E2 on Ishikawa cells NRP-1 protein expression in a time-dependent manner. CONCLUSIONS: NRP-1 is expressed in Ishikawa cells with high expression of estrogen receptor (ER) and HEC-1A cells with low expression of ER. HUVECs treated with 2 × 10-7mol / L E2 for 48h can significantly promote the expression of NRP- 1 expression, suggesting that NRP-1 may be closely related to the occurrence and development of endometrial cancer.