本实验,目的在于在体外培养条件下复制出相当于活体内骨髓“间质性细胞”微环境对造血细胞的影响。妊娠10～11天以后的小鼠胚胎肝脏是个重要的造血器官,生后1周造血功能停止。在成年小鼠肝内存在有造血先质(祖)细胞。它们可能是来自血中的CFUs移植到支持它们生长的肝脏微环境,或者可能来自胚胎肝内持续性存在的CFUs在肝内激活。胚胎肝脏及成年期肝脏在体内提供造血细胞增殖的良好微环境。本文用小鼠胚胎肝、成年小鼠脾脏、骨髓及胚胎成纤维细胞来源的底细胞层(贴附细胞层,Adheremt Cell Layer)作为条件培养基Condition Mdiun,(M)来观察骨髓 The purpose of this experiment is to replicate the effect of microenvironment corresponding to the “interstitial cells” of bone marrow in vivo on the hematopoietic cells in vitro. Mouse embryonic liver 10 to 11 days after pregnancy is an important hematopoietic organ, one week after birth, hematopoietic function stops. Hematopoietic progenitor (progenitor) cells exist in the liver of adult mice. They may be transplanted with CFUs from the blood into the liver microenvironment that supports their growth or may be activated intrahepaticly by CFUs that persist from within the embryonic liver. Embryonic livers and adult livers provide a good microenvironment for hematopoietic cell proliferation in vivo. In this paper, we used mouse embryonic liver, adult mouse spleen, bone marrow and embryonic fibroblast-derived basal cell layer (Adheremt Cell Layer) as a conditioned medium Condition Mdiun, (M) to observe the bone marrow