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目的 探讨蝙蝠葛碱 (DAU )对急性心肌梗死 (AMI)患者血小板不可逆性聚集的作用。方法 用流式细胞仪测定AMI患者 (n =12 )静息状态及凝血酶 (5 0、10 0、5 0 0、10 0 0U·L-1)激活时 ,血小板膜表面糖蛋白Ⅳ (GPIV)及凝血酶敏感蛋白 (TSP )分布状况 ,并与健康人 (n =14)比较。结果 AMI患者静息及激活状态血小板膜表面GPⅣ ,TSP分布高于健康人 (P <0 0 5 ,0 0 1) ;DAU(5 0 μmol·L-1)可抑制 5 0U·L-1,10 0U·L-1及 5 0 0U·L-1凝血酶诱导的血小板膜GPⅣ再分布及血小板内TSP释放 (P <0 0 5 ,0 0 1) ,不能抑制10 0 0U·L-1凝血酶诱导的血小板膜GPⅣ再分布及血小板内TSP释放。结论 AMI患者血小板活性及对凝血酶的反应性增高 ,DAU可减少AMI患者血小板不可逆性聚集的发生。
Objective To investigate the effect of dauricine on irreversible aggregation of platelets in patients with acute myocardial infarction (AMI). Methods Flow cytometry was used to detect platelet membrane glycoprotein Ⅳ (GPIV) activity in resting AMI patients (n = 12) and thrombin (50, 100, 0, 100, 100U · L-1) ) And thrombospondin (TSP), and compared with healthy people (n = 14). Results The distribution of GPIV and TSP on resting and activated platelets in AMI patients was higher than that in healthy volunteers (P <0.05, 0.01). DAU (50 μmol·L-1) Platelet membrane GPⅣ redistribution induced by 10 U · L-1 and 500 U · L-1 thrombin and platelet TSP release (P <0.05, 0.01) did not inhibit clotting of 100 U · L-1 Enzyme - induced platelet membrane GPIV redistribution and platelet TSP release. Conclusion Platelet activity and reactivity to thrombin in patients with AMI are increased. DAU can reduce the irreversible aggregation of platelets in AMI patients.