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采用鱼类毒性试验方法，测定增效剂B002和杀螺剂氯硝柳胺及B002和氯硝柳脸（1：1）复方对草鱼、鲢鱼的急性毒性。结果表明B002为200mg/L时48h，96h对草、鲢鱼无急性毒性，属低毒药物；氯硝柳胺对草鱼和鲢鱼48，96h的TLM值为0．1192和0．1777mg/L属剧毒药物；B002和氯硝柳胺复方对草鱼和链鱼的48，96h的TLM值为0．3124和0．3027mg/L.因此，B002并不增加氯硝柳胺对草鱼、鲢鱼的毒性，对鱼毒性无增效作用。
Acute toxicity to grass carp and silver carp was determined by the toxicity test of fish with the synergist B002 and the snailing agents niclosamide and B002 and sildenafil face (1: 1). The results showed that B002 200mg / L at 48h, 96h grass, silver carp no acute toxicity, is a low-toxic drugs; niclosamide on grass carp and silver carp 48,96h TLM values of 0.1192 and 0.1777mg / L Is a highly toxic drug; B002 and niclosamide compound on grass carp and chain fish 48,96h TLM values of 0.3124 and 0.3027mg / L. Therefore, B002 does not increase niclosamide on grass carp, silver carp Toxicity, no synergistic effect on fish toxicity.